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KMID : 0545120120220040530
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Journal of Microbiology and Biotechnology
2012 Volume.22 No. 4 p.530 ~ p.534
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Development of Real-Time PCR for the Detection of Clostridium perfringens in Meats and Vegetables
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Chon Jung-Whan
Park Jong-Seok
Hyeon Ji-Yeon
Park Chan-Kyu
Song Kwang-Young
Hong Kwang-Won
Hwang In-Gyun
Kwak Hyo-Sun
Seo Kun-Ho
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Abstract
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A real-time PCR assay was developed and validated inhouse specifically for the detection of Clostridium perfringens (Cl. perfringens) in meats and vegetables by comparing with the culture method. The detection limit of the real-time PCR assay in phosphate-buffered saline was 102 CFU/ml. When the two methods were compared in food samples inoculated with Cl. perfringens, the culture method detected 52 positives, whereas real-time PCR detected 51 positives out of 160 samples. The difference was without statistical significance (p>0.05). Real-time PCR assay is an option for quality assurance laboratories to perform standard diagnostic tests, considering its detection ability and time-saving efficiency.
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KEYWORD
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Clostridium perfringens, real-time PCR, culture method, foods, detection
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